This shows the virility strength for bulls. The V-shape of the microfluidic probe allow researchers to count the number of sperm swimming upstream in a simulated reproductive tract. Freezing with cryoprotectant 6% G yielded the best post-thaw sperm characteristics for Eastern Anatolian Red bull spermatozoa.Ĭopyright © 2012 Elsevier Inc. Soon, a quick and easy method reminiscent of a home pregnancy test can tell if a breeding bull has the right stuff. In conclusion, no advantages were found in using EG or DMSO to replace G in bull sperm cryopreservation. The antioxidant activities of GPx, GSH, and CAT as well as the total antioxidant activity were affected by the type of cryoprotectant notably, 2% G+2% EG+2% DMSO yielded the lowest activities when compared to the other groups (P<0.001). 6% DMSO and 3% EG + 3% DMSO resulted in greater chromatin damage than the other groups (P<0.001). 6% G and 6% EG showed the greatest values of preserved membrane integrity (P<0.001). 6% G exhibited the greatest percentages of CASA (43.7☒.92%) and progressive (26.4☒.64%) motilities when compared to the other groups (P<0.001). DNA damage was evaluated by Comet Assay using Image Analysis System. Biochemical assays were performed in a spectrophotometer using commercial kits. Frozen straws were thawed individually at 37☌ for 30s in a water bath to analyse progressive motility and sperm motion characteristics as well as membrane integrity using hypo-osmotic swelling test. Thereafter, the straws were plunged into liquid nitrogen at -196☌. VikingGenetics Experts in bull mating and bull semen The perfect bull and bull semen is just a few options away Look through our bull catalogue. They were then loaded into 0.25ml French straws and frozen using a digital freezing machine at 3 programmed rates: -3☌/min from +4☌ to -10☌, -40☌/min from -10☌ to -100☌, and -20☌/min from -100☌ to -140☌. The extended samples were cooled slowly to 4☌ and equilibrated for 4h. The pooled ejaculates were also split into seven equal experimental groups and diluted with the modified base extender to a final spermatozoa concentration of 15×10(6)/ml. The ejaculates were pooled to increase the semen volume for replication and to eliminate variability among the evaluated samples. Ejaculates were collected from the three bulls using an artificial vagina twice a week. The objectives of this study was to compare the effects of type and concentration of cryoprotectants glycerol (G), ethylene glycol (EG) and dimethyl sulfoxide (DMSO) on the plasma membrane and DNA integrity as well as antioxidant activity of cryopreserved Eastern Anatolian red bull sperm.
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